Cover image

Single-molecule imaging of quantum dot-labeled kinesin motors on surface-immobilized microtubule filaments. The various colors illustrate the processive movement of individual kinesin motors toward the fast-growing ends of microtubules over time. In this issue, Ahmet Yildiz and Ronald Vale describe methods for achieving sensitive detection of weak fluorescent signals from single molecules using total internal reflection illumination (doi: 10.1101/pdb.top086348). Image by Ahmet Yildiz.