Recipe

Gradient Buffer A

5 mm 2-(N-morpholino)ethanesulfonic acid

1 mm EDTA

1 mm KCl

8% (w/v) sucrose

0.1% (v/v) ethanol

Adjust to pH 6 at room temperature with KOH.

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  1. doi:10.1101/pdb.rec084434 Cold Spring Harb Protoc 2015: pdb.rec084434- © 2015 Cold Spring Harbor Laboratory Press
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