Recipe

Sorbitol Buffer for Spheroplasts

1.2 m sorbitol

20 mm K3PO4

Adjust pH to 7.4 at room temperature with 1 m KH2PO4.

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  1. doi:10.1101/pdb.rec084491 Cold Spring Harb Protoc 2015: pdb.rec084491- © 2015 Cold Spring Harbor Laboratory Press
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