Recipe

YNO Medium

Reagent 1× 10×
Yeast nitrogen base (w/v) 0.67% 6.7%
Yeast extract  0.1%   1%
Oleic acid (v/v)  0.1%   1%
Tween 40 (v/v) 0.02% 0.2%
Adjust to pH 6 at room temperature using KOH.
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  1. doi:10.1101/pdb.rec084525 Cold Spring Harb Protoc 2015: pdb.rec084525- © 2015 Cold Spring Harbor Laboratory Press
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