| Bacto peptone |
20 g |
2% (w/v) |
| Yeast extract |
10 g |
1% (w/v) |
| Dextrose |
20 g |
2% (w/v) |
| Agar (optional) |
20 g |
2% (w/v) |
| G418 (200 mg/mL; optional) |
1 mL |
200 mg/L |
| Add 1 L of deionized water to 20 g bacto peptone, 10 g yeast extract, and 20 g dextrose (and 20 g of agar for YEPD plates).
Sterilize by autoclaving for 20 min at 15 psi (1.05 kg/cm2). To prepare YEPD plus G418 plates, allow the solution to cool to ∼60°C, add 1 mL of G418 stock solution, and pour into Petri
dishes (∼25 mL per 100-mm plate). Store YEPD medium without G418 at room temperature, and store YEPD containing medium G418
at 4°C.
|
