Supplements for SD-Lys
| Reagent | Quantity | |
|---|---|---|
| l-Leucine | 0.84 g | |
| l-Histidine HCl | 0.42 g | |
| l-Methionine | 0.42 g | |
| Uracil | 0.25 g | |
| In separate tubes, prepare 100× stocks of each of the four reagents by dissolving the quantities indicated in 100 mL of deionized water and then filter-sterilizing. Store all stocks at room temperature; they will keep for at least 6 mo. | ||
- © 2015 Cold Spring Harbor Laboratory Press
