Resuspension Buffer for Cellular DNA Preparation
| Reagent | Quantity (for 100 mL) |
Final concentration |
|---|---|---|
| Tris (1 m, pH 8.0) | 2 mL | 20 mm |
| EDTA (0.5 m, pH 8.0) | 0.2 mL | 1 mm |
| NaCl (5 m) | 0.2 mL | 10 mm |
| Triton X-100 (10%, w/v) | 1 mL | 0.1% (w/v) |
| H2O | 96.6 mL | |
| Store indefinitely at 4°C. | ||
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