Cytoskeletal (CSK) Buffer
| Reagent | Quantity (for 50 mL) | Final concentration |
|---|---|---|
| PIPES/KOH (1 m, pH 6.8) | 0.5 mL | 10 mm |
| NaCl (5 m) | 1 mL | 100 mm |
| Sucrose | 5.135 g | 300 mm |
| EGTA (250 mm) | 0.2 mL | 1 mm |
| MgCl2 (1 m) | 50 µL | 1 mm |
| DTT (1 m) | 50 µL | 1 mm |
| Protease inhibitor cocktail (e.g., Roche 05056489001) | 1 tablet | |
| Adjust the volume to 50 mL with distilled H2O and filter-sterilize. Store in aliquots at −20°C. Add the DTT and protease inhibitor cocktail tablet immediately before use. | ||
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