Characterization of Protein–Protein Interactions Using Protein Microarrays

Abstract

Functional protein microarrays allow fast, straightforward, and efficient high-throughput screening of protein–protein interactions. The microarray approach has outpaced other interaction methods, such as yeast two-hybrid screens, in part because of the vast amounts of information that can be obtained during a single assay. This protocol describes how to perform a binding assay for a protein of interest using a proteome microarray composed of thousands of functional, recombinant proteins adhered to a microchip.