Preparation of Plasmid DNA by Alkaline Lysis with Sodium Dodecyl Sulfate: Minipreps
Abstract
In this protocol, plasmid DNA is isolated from small-scale (1–2 mL) bacterial cultures. Yields vary between 100 and 5 µg of DNA, depending on the copy number of the plasmid. Miniprep DNA is sufficiently pure for use as a substrate or template in many in vitro enzymatic reactions. However, further purification is required if the plasmid DNA is used as the substrate in sequencing reactions.
Footnotes
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From the Molecular Cloning collection, edited by Michael R. Green and Joseph Sambrook.
- © 2016 Cold Spring Harbor Laboratory Press
