Protein Microarrays: Flexible Tools for Scientific Innovation
- Johnathan Neiswinger1,2,
- Ijeoma Uzoma1,2,
- Eric Cox1,2,3,
- HeeSool Rho1,2,
- Guang Song1,2,
- Corry Paul1,2,
- Jun Seop Jeong1,2,
- Kuan-Yi Lu4,
- Chien-Sheng Chen4 and
- Heng Zhu1,2,5,6
- 1Department of Pharmacology and Molecular Sciences, Johns Hopkins School of Medicine, Baltimore, Maryland 21287;
- 2The Center for High-Throughput Biology, Johns Hopkins School of Medicine, Baltimore, Maryland 21287;
- 3Department of Neuroscience, Johns Hopkins School of Medicine, Baltimore, Maryland 21287;
- 4Graduate Institute of Systems Biology and Bioinformatics, National Central University, Jhongli 32001, Taiwan;
- 5The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins School of Medicine, Baltimore, Maryland 21287
Abstract
Protein microarrays have emerged as a powerful tool for the scientific community, and their greatest advantage lies in the fact that thousands of reactions can be performed in a parallel and unbiased manner. The first high-density protein microarray, dubbed the “yeast proteome array,” consisted of approximately 5800 full-length yeast proteins and was initially used to identify protein–lipid interactions. Further assays were subsequently developed to allow measurement of protein–DNA, protein–RNA, and protein–protein interactions, as well as four well-known posttranslational modifications: phosphorylation, acetylation, ubiquitylation, and SUMOylation. In this introduction, we describe the advent of high-density protein microarrays, as well as current methods for assessing a wide variety of protein interactions and posttranslational modifications.
Footnotes
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↵6 Correspondence: heng.zhu{at}jhmi.edu
- © 2016 Cold Spring Harbor Laboratory Press
