TABLE 1.
Plasmid and oligonucleotide list (Tarassov et al. 2008)
| Plasmid or oligonucleotide | Characteristics |
|---|---|
| pAG25-linker-DHFR-F[1,2] Antibiotic resistance: nourseothricin pAG32-linker-DHFR-F[3] Antibiotic resistance: hygromycin-B |
For creating integration cassettes of DHFR PCA fragments to study yeast PPIs at endogenous levels of expression. Cassettes are integrated by homologous recombination. |
| Forward oligonucleotides for creating integration cassettes | 61 base pairs long, consisting of a 40-nucleotide sequence homologous to a region located 5′ to the ORF stop codon, followed by a 21-nucleotide sequence that anneals to the linker: 5′-GGCGGTGGCGGATCAGGAGGC-3′ |
| Reverse oligonucleotides for creating integration cassettes | 64 base pairs long, consisting of a 40-nucleotide sequence homologous to the sequence of genomic DNA immediately 3′ to the stop codon of each ORF, followed by a 24-nucleotide sequence that anneals to the 3′ end of the TEF terminator region of the antibiotic resistance cassettes: 5′-TTCGACACTGGATGGCGGCGTTAG-3′ |
