Recipe

Amino Acid Mix (10×)

Amino acid or nucleobase Concentration (10×)
Adenine sulfate  0.4 g/L
Uracil  0.2 g/L
l-Tryptophan  0.4 g/L
l-Histidine hydrochloride  0.2 g/L
l-Arginine hydrochloride  0.2 g/L
l-Tyrosine  0.3 g/L
l-Leucine  0.6 g/L
l-Lysine hydrochloride  0.3 g/L
l-Phenylalanine  0.5 g/L
l-Glutamic acid  1.0 g/L
l-Asparagine  1.0 g/L
l-Valine  1.5 g/L
l-Threonine  2.0 g/L
l-Serine 3.75 g/L
l-Methionine  0.2 g/L
Dissolve the amino acids from the list above (except those to be excluded from any drop-out media) in distilled H2O. Filter-sterilize, store at 4°C, and protect from light.
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  1. doi:10.1101/pdb.rec090308 Cold Spring Harb Protoc 2016: pdb.rec090308- © 2016 Cold Spring Harbor Laboratory Press

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