Recipe

Synthetic Complete (SC) Medium

Reagents Final concentration (1×)
Yeast nitrogen base without amino acids and with ammonium sulfate   6.7 g/L
Agar 2% (w/v)
Amino acid mix (10×)    1×
Glucose 2% (w/v)
Store synthetic complete medium for up to several months at 4°C.
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  1. doi:10.1101/pdb.rec090589 Cold Spring Harb Protoc 2016: pdb.rec090589- © 2016 Cold Spring Harbor Laboratory Press

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