Spo.a and Spo.α Sporulation Media
| Reagents | Final concentration (1×) |
|---|---|
| Potassium acetate | 10 g/L |
| Yeast extract | 1 g/L |
| Agar | 2% (w/v) |
| Uracil, histidine, leucine, and lysine mix at a mass ratio of 1:1:5:1 | 0.1 g/L |
| Glucose | 0.5 g/L |
| Geneticin | 50 µg/mL |
| Nourseothricin (for spo.a medium only) | 25 µg/mL |
| Hygromycin B (for spo.α medium only) | 62.5 µg/mL |
| Store for up to several months at 4°C. | |
- © 2016 Cold Spring Harbor Laboratory Press
