iCycler Mix (2×)
| Reagent | Volume/final concentration (for 1 mL) |
|---|---|
| PCR buffer (10×; 200 mm Tris-HCl, 500 mm KCl, pH 8.4) | 200 µL |
| MgCl2 | 25 mm |
| dNTPs | 10 mm |
| Sterilize using a disposable 0.22-µm vacuum filtration system in a tissue-culture hood and store at room temperature. | |
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