Recipe

Proteinase K Incubation Buffer (2×)

Reagent Final concentration
Tris-HCl (pH 8.0) 10 mm
Na2EDTA 20 mm
NaCl2 20 mm
Sterilize using a disposable 0.22-µm vacuum filtration system in a tissue-culture hood and store at room temperature.
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  1. doi:10.1101/pdb.rec091108 Cold Spring Harb Protoc 2016: pdb.rec091108- © 2016 Cold Spring Harbor Laboratory Press
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