Recipe

Iodixanol Solutions (15%, 24%, and 40%)

Reagent Volume (for 15%) Volume (for 24%) Volume (for 40%)
Iodixanol solution (54%) 4.72 mL 5.56 mL 7.41 mL
PBS-MK (1×, containing 2 m NaCl) 8.50 mL – –
PBS-MK (1×, containing 0.137 m NaCl) 3.78 mL 6.44 mL 2.59 mL
Phenol red (0.5%) –   60 µL –
Total   17 mL ∼ 12 mL   10 mL
Sterilize each solution using a disposable 0.22-µm vacuum filtration system in a tissue-culture hood and store at room temperature.
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  1. doi:10.1101/pdb.rec091116 Cold Spring Harb Protoc 2016: pdb.rec091116- © 2016 Cold Spring Harbor Laboratory Press
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