Recipe

Iodixanol Solution (54%)

Reagent Volume to add
OptiPrep Density Gradient Medium (60% iodixanol;  Sigma-Aldrich D1556)  40 mL
PBS-MK (10×, containing 1.37 m NaCl) 4.44 mL
Sterilize the 54% iodixanol solution using a disposable 0.22-µm vacuum filtration system in a tissue-culture hood and store at room temperature. Add 60 µL of 0.5% phenol red to a 12-mL aliquot of the 54% iodixanol solution.
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  1. doi:10.1101/pdb.rec091140 Cold Spring Harb Protoc 2016: pdb.rec091140- © 2016 Cold Spring Harbor Laboratory Press
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