| Peptone |
40 g |
| Yeast extract |
20 g |
| Adenine hemisulfate dehydrate |
0.32 g |
| Glucose (40%, w/v) in water, sterile |
100 mL |
| Agar |
35 g |
| Dissolve the first three powders in 950 mL of water in a 2-L flask and add a stir bar. In a second 2-L flask, add the agar
to 950 mL of water (do not add a stir bar as it will cause the agar to boil over in the autoclave). Autoclave for 40 min at
15 psi on liquid cycle. Immediately pour the contents of the first flask, including the stir bar, into the agar-containing
flask. Add the glucose, mix well on a stir plate, and cool to 55°C. Pour into 150-mm sterile Petri dishes (∼80 mL per dish),
dry for 3–5 d at room temperature, wrap in plastic bags, and store for up to 6 mo at room temperature.
|
