Tissue Dissociation for Metastasis Studies
- 1Institut de Recherches Cliniques de Montréal, Quebec H2W 1R7, Canada;
- 2Pathologie et Biologie Cellulaire, Université de Montréal, Quebec H3T 1J4, Canada;
- 3Department of Medicine, Experimental Medicine Division, McGill University, Montréal, Quebec H3A 13A, Canada
Abstract
The main requirement for most metastasis-related applications is the conversion of solid tissue into a single-cell suspension. In theory, this suspension represents the diversity of cells present in the tissue, whether malignant or benign. We have found that cell viability, as measured by trypan blue staining or fluorescence-activated cell sorting (FACS), is critical for evaluating the success of the tissue-dissociation procedure. The recommended goal is at least 70% cell viability.
Footnotes
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↵4 Correspondence: farhia.kabeer{at}ircm.qc.ca
- © 2016 Cold Spring Harbor Laboratory Press
