Overview of the calling card protocol. (A) Barcoded Ty5 transposons are separately transformed into yeast strains with TFs tagged with Sir4. (B) The strains are pooled, expression of Ty5 is induced, and the subsequent growth medium selects for genomic insertions. (C) Genomic DNA is digested with restriction endonucleases. (D) Digested fragments are self-ligated. (E) Inverse PCR adds Illumina sequencing adaptors.