Recipe

Digestion Solution for Tissue Dissociation

10 mL collagenase/hyaluronidase in DMEM (10×; StemCell Technologies 07912)

90 mL complete medium for metastasis studies <R>

Filter-sterilize using a 0.22-μm filter. Aliquot and store for up to 2 mo at −20°C.

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  1. doi:10.1101/pdb.rec079079 Cold Spring Harb Protoc 2016: pdb.rec079079- © 2016 Cold Spring Harbor Laboratory Press
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