Recipe

HEPES-Buffered Saline (HBS) Precipitation Buffer (2×)

50 mm HEPES

280 mm NaCl

Adjust the pH to 7.10 (±0.05) with NaOH. Bring the final volume to 1 L in ddH2O. Filter-sterilize. Store at 25°C for up to 12 mo.

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  1. doi:10.1101/pdb.rec079194 Cold Spring Harb Protoc 2016: pdb.rec079194- © 2016 Cold Spring Harbor Laboratory Press

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