Laemmli Sample Buffer (LSB)
| Final concentration | |||
|---|---|---|---|
| Reagent | 4× LSB | 2× LSB | 1× LSB |
| Tris–HCl (pH 6.8) | 200 mm | 100 mm | 50 mm |
| SDS | 4% (w/v) | 4% (w/v) | 4% (w/v) |
| Glycerol | 40% (v/v) | 20% (v/v) | 10% (v/v) |
| Bromophenol blue | 0.4% (w/v) | 0.2% (w/v) | 0.1% (w/v) |
| β-Mercaptoethanol | 20% (v/v) | 10% (v/v) | 5% (v/v) |
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