Recipe

Glucose − His 5-Fluoroorotic Acid (5-FOA) Agar Plates

1.7 g Yeast nitrogen base without amino acids and ammonium sulfate
  1 g Drop-out mix (without histidine) <R>
  5 g Ammonium sulfate
 20 g Agar
Add H2O to 900 mL, stir well, and autoclave. Add 100 mL of 20% glucose (sterile) and 1 g 5-FOA, and stir. Pour into sterile Petri dishes and allow to solidify.
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  1. doi:10.1101/pdb.rec087510 Cold Spring Harb Protoc 2016: pdb.rec087510- © 2016 Cold Spring Harbor Laboratory Press
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