Recipe

Glucose − Ura Medium

1.7 g Yeast nitrogen base without amino acids and ammonium sulfate
  1 g Drop-out mix (without uracil) <R>
  5 g Ammonium sulfate
Add H2O to 900 mL, stir well, and autoclave. Add 100 mL of 20% glucose (sterile) and stir. Store for up to 3 mo at 4°C.
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  1. doi:10.1101/pdb.rec087726 Cold Spring Harb Protoc 2016: pdb.rec087726- © 2016 Cold Spring Harbor Laboratory Press
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