Yeast Extract with Supplements (YES) and Phloxin B
| Reagent | Amount to add (1 L) |
|---|---|
| Glucose | 30 g |
| Yeast extract | 5 g |
| Adenine | 0.2 g |
| Uracil | 0.2 g |
| Histidine | 0.2 g |
| Leucine | 0.2 g |
| Lysine | 0.2 g |
| Agar (for solid medium only) | 20 g |
| H2O | to 1 L |
| Prepare 1 L of YES by combining the reagents listed above. Sterilize by autoclaving at 10 psi for 15 min. Add 4 mL of Phloxin B stock solution (5 g/L, filter-sterilized) to 1 L of YES after autoclaving. Store at 4°C. (Note that the yeast extract contains sufficient thiamine to repress nmt1 promoters.) | |
- © 2016 Cold Spring Harbor Laboratory Press
