β-Hexosaminidase Substrate Solution
| Reagent | Amount (for 100 mL) | Final concentration |
|---|---|---|
| Citrate | 100 mm (pH 5) | |
| Na3-citrate | 1.91 g | |
| Citrate | 0.72 g | |
| Substrate (4-methylumbelliferyl-2-acetamido-2-deoxy-β-d-glucopyranoside; Sigma-Aldrich M2133) | 19 mg | 0.5 mm |
| Sucrose | 8.6 g | 0.27 m |
| Aliquot and store at −20°C. | ||
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