Recipe

Protoplast Transformation Solution D

Tris–HCl 10 mm, pH 7.6
Sorbitol     1.2 m
CaCl2    10 mm
Sterilize by autoclaving.
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  1. doi:10.1101/pdb.rec092239 Cold Spring Harb Protoc 2016: pdb.rec092239- © 2016 Cold Spring Harbor Laboratory Press
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