SOS Buffer
| Tris–HCl | 10 mm, pH 7.6 |
| CaCl2 | 10 mm |
| Sorbitol | 1.2 m |
| Yeast extract | 0.5 mg/mL |
| Leucine | 5 µg/mL |
| Uracil | 5 µg/mL |
| Adenine | 5 µg/mL |
| Arginine | 5 µg/mL |
| Histidine | 5 µg/mL |
| Combine components in distilled water and filter-sterilize. Make fresh for each transformation. | |
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