Culture Medium for SCG Neurons
| Reagent | Final concentration |
|---|---|
| Minimum essential medium with Earle’s salts and l-glutamine (MEM; e.g., Life Technologies 11095) | 90% |
| Fetal bovine serum | 10% |
| Penicillin | 500 U/mL |
| Streptomycin | 500 µg/mL |
| Prepare medium under sterile conditions. Divide into 50-mL aliquots. Store at 4°C for up to 1 mo. | |
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