| Methanesulfonic acid (CH3SO3H)
|
140 mm |
| Tetraethylammonium hydroxide (TEA-OH) |
145 mm |
| HEPES |
10 mm |
| d-glucose
|
15 mm |
| CaCl2·2H2O
|
10 mm |
| Tetrodotoxin (TTX) |
0.0003 mm |
| Prepare solution containing all but TTX in deionized water. Adjust pH to 7.4 with TEA-OH. (Osmolality should range between
310 and 325 mmol/kg.) Filter using a 0.2-µm-pore membrane filter. Store up to 3 mo at room temperature (20°C–22°C) or up to
6 mo at 4°C.
|
| Add TTX before the start of recordings. Be aware that TTX is susceptible to breakdown under basic (high-pH) conditions. |
