| HCl |
20 mm |
| N-methyl-d-glucamine (NMG)
|
120 mm |
| Tetraethylammonium hydroxide (TEA-OH) |
20 mm |
| EGTA |
11 mm |
| HEPES |
10 mm |
| Sucrose |
10 mm |
| CaCl2 |
1 mm |
| Tris-creatine phosphate |
14 mm |
| Mg-ATP |
4 mm |
| Na2-GTP
|
0.3 mm |
| Prepare in deionized water. Adjust pH to ~8.5 with methanesulfonic acid (CH3SO3H) before adding Tris-creatine phosphate, Mg-ATP, and Na2-GTP. Adjust final pH to 7.2 with methanesulfonic acid (CH3SO3H). (Osmolality should range between 290 and 305 mmol/kg.) Filter internal recording solution using a 0.2-µm-pore membrane
filter. Divide into 5-mL aliquots and store up to 6 mo at −20°C.
|
