Recipe

Spheroplast Buffer

Sorbitol      0.6 m
KH2PO4/K2HPO4 50 mm, pH 7.5
Combine components and filter-sterilize. (It is important that the buffer does not contain either EDTA or citrate.)
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  1. doi:10.1101/pdb.rec092163 Cold Spring Harb Protoc 2016: pdb.rec092163- © 2016 Cold Spring Harbor Laboratory Press
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