(A) The configuration of the elutriation system. Input into the pump can be switched between a reservoir bottle containing medium and the 5-L flask containing culture. The bubble trap is called the “sample reservoir” in the manual from Beckman Coulter. The exhaust tube from the rotor (red) is taped to the top of an empty bottle inside a drip tray during elution to ensure the height of the tube remains fixed, as the simple act of raising or lowering the height of the tube will move the front back and forth on the rotor. (B) Depictions of the appearance of the cells in the 40-mL chamber at the indicated stages of the protocol. (C) Frequency of septation and pre- and post-NETO cells as size-selected cells transit two consecutive cell cycles. NETO is delayed in the G2 population isolated by size selection.
