| Prepare the following 10× stock solution in double-distilled water (ddH2O). Store it at 4°C for a maximum of 1 wk.
|
| NaCl |
1250 mm |
| KCl |
25 mm |
| NaH2PO4 |
12.5 mm |
| NaHCO3 |
260 mm |
| On the day of the experiment, dilute the 10× solution 10-fold, bubble for 10 min with carbogen (95% O2/5% CO2), and supplement with the following.
|
| MgCl2 |
1 mm |
| CaCl2 |
2 mm |
| Glucose |
~26 mm |
| ACSF—in particular Ca2+ and Mg2+ concentrations—may be varied depending on what is to be studied. For example, higher Ca2+ concentration favors long-term potentiation (LTP), whereas lower Ca2+ concentration promotes long-term depression (LTD). Adjust osmolality to ~320 mOsm with d-glucose for rat and ~338 mOsm for mouse.
|
