Recipe

SPO Agar

  • 1. Combine the following reagents in deionized H2O.

    Reagent Final concentration
    Agar 2%
    Potassium acetate 2%

  • 2. Sterilize by autoclaving for 20 min at 15 psi.

  • 3. Add trace nutrients appropriate for the yeast background of interest.

  • 4. Pour 20–25 mL of agar medium per 90 × 15-mm Petri dish.

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  1. doi:10.1101/pdb.rec086074 Cold Spring Harb Protoc 2016: pdb.rec086074- © 2016 Cold Spring Harbor Laboratory Press
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