Recipe

Agar (2×)

15 g agar

1 L water

Sterilize by autoclaving for 20 min at 15 psi (1.05 kg/cm2) on liquid cycle. Swirl gently to distribute the melted agar evenly throughout the solution. Be careful; the fluid may become superheated and may boil over when swirled. Store for up to 6 mo at 23°C.

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  1. doi:10.1101/pdb.rec089599 Cold Spring Harb Protoc 2016: pdb.rec089599- © 2016 Cold Spring Harbor Laboratory Press

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