YPD (1× and 2×)
| 1× YPD | 2× YPD | |
|---|---|---|
| Glucose (20% in water) | 100 mL | 200 mL |
| Peptone | 20 g | 40 g |
| Yeast extract | 10 g | 20 g |
| Water | 900 mL | 800 mL |
| Dissolve all components in the water and sterilize by autoclaving for 20 min at 15 psi (1.05 kg/cm2) on liquid cycle. Store for up to 1 yr at room temperature. | ||
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