GenFlex Tag 16K 2× Hybridization Buffer
First prepare ∼5 mL of 0.66 m MES hydrate (Sigma-Aldrich M5287) and ∼5 mL of 1.78 m MES sodium salt (Sigma-Aldrich M5057). Mix equal volumes of each to create a 12× MES stock solution. Use this to make 50 mL of 2× hybridization buffer as follows.
| Reagent | Amount | Final concentration |
|---|---|---|
| MES stock (12×) | 8.3 mL | 2× |
| NaCl (5 m) | 17.7 mL | 1.77 m |
| EDTA (500 mm) | 4 mL | 40 mm |
| Tween 20 (10%) | 100 µL | 0.02% |
| Deionized water | 19.9 mL | |
| Store buffer for up to 3 mo at 4°C. | ||
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