GenFlex Tag16K Tag Hybridization Mixture

Recipe

Reagent	Amount	Final concentration
GenFlex Tag 16K 2× Hybridization Buffer <R>	74.5 µL	1.7×
B213 oligonucleotide (Biotin-
CTGAACGGTAGCATCTTGAC) (2 μm)	0.5 µL	11 nm
Blocking oligonucleotides (12.5 µm stocks):
Uptag (GATGTCCACGAGGTCTCT)	1.5 µL	0.2 µm
Dntag (CGGTGTCGGTCTCGTAG)	1.5 µL	0.2 µm
Uptagkanmx (GTCGACCTGCAGCGTACG)	1.5 µL	0.2 µm
Dntagkanmx (CGAGCTCGAATTCATCG)	1.5 µL	0.2 µm
Uptagcomp (AGAGACCTCGTGGACATC)	1.5 µL	0.2 µm
Dntagcomp (CTACGAGACCGACACCG)	1.5 µL	0.2 µm
Upkancomp (CGTACGCTGCAGGTCGAC)	1.5 µL	0.2 µm
Dnkancomp (CGATGAATTCGAGCTCG)	1.5 µL	0.2 µm
Denhardt’s (50×) (Sigma-Aldrich D2532)	3 µL	1.7×
For each sample, combine the above for a total of 90 µL. Prepare immediately before use.