Recipe

Extracellular Recording Solution

Reagent Concentration
CaCl2 or BaCl2  10 mm
MgCl2  1 mm
Tris 150 mm
Prepare Ca2+- and Ba2+-containing variants of the solution to measure Ca2+-dependent inactivation and voltage-dependent inactivation, respectively, of voltage-gated Ca2+ channels. Adjust pH to 7.3 using methanesulfonic acid. (The osmolarity should be ~290–310.) Filter and store at 4°C.
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  1. doi:10.1101/pdb.rec094201 Cold Spring Harb Protoc 2016: pdb.rec094201- © 2016 Cold Spring Harbor Laboratory Press
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