Protocol

Isolating DNA from Gram-Negative Bacteria

  1. Joseph Sambrook

Abstract

The isolation of DNA from bacteria, described in this protocol, relies upon the use of sodium dodecyl sulfate and proteinase K to lyse the cells. High-molecular-weight DNA is then sheared (to reduce its viscosity and make it more manageable), extracted with phenol:chloroform, and precipitated with isopropanol. DNA isolated according to this procedure ranges from 30 to 80 kb in length.

Footnotes

  • From the Molecular Cloning collection, edited by Michael R. Green and Joseph Sambrook.

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  1. doi:10.1101/pdb.prot093369 Cold Spring Harb Protoc 2017: pdb.prot093369- © 2017 Cold Spring Harbor Laboratory Press
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