Isolating DNA from Gram-Negative Bacteria
Abstract
The isolation of DNA from bacteria, described in this protocol, relies upon the use of sodium dodecyl sulfate and proteinase K to lyse the cells. High-molecular-weight DNA is then sheared (to reduce its viscosity and make it more manageable), extracted with phenol:chloroform, and precipitated with isopropanol. DNA isolated according to this procedure ranges from 30 to 80 kb in length.
Footnotes
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From the Molecular Cloning collection, edited by Michael R. Green and Joseph Sambrook.
- © 2017 Cold Spring Harbor Laboratory Press
