Protocol

Whole-Genome Sequencing: Manual Library Preparation

  1. W. Richard McCombie

Abstract

This protocol describes a manual approach for the preparation of genomic DNA libraries suitable for Illumina sequencing. Genomic DNA fragments produced by shearing by sonication are ligated to adaptors and amplified by polymerase chain reaction (PCR). The amplified DNA, separated by size and gel-purified, is suitable for use as template in whole-genome sequencing.

Footnotes

  • From the Molecular Cloning collection, edited by Michael R. Green and Joseph Sambrook.

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This Article

  1. Published in Advance November 1, 2016, doi: 10.1101/pdb.prot094615 Cold Spring Harb Protoc 2017: pdb.prot094615- © 2017 Cold Spring Harbor Laboratory Press
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