Recipe

Freeze-Substitution Cocktail

Acetone

2% osmium tetroxide

0.1% uranyl acetate

Dissolve osmium tetroxide and uranyl acetate in 100% acetone. Prepare the solution in a fume hood; osmium tetroxide is volatile and highly reactive.

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  1. doi:10.1101/pdb.rec092148 Cold Spring Harb Protoc 2017: pdb.rec092148- © 2017 Cold Spring Harbor Laboratory Press
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