| CsF |
1.329 g |
35 mm
|
| CsCl |
4.209 g |
100 mm
|
| EGTA |
0.951 g |
10 mm
|
| HEPES |
0.596 g |
10 mm
|
| In a 250-mL volumetric flask, combine the above reagents. Add ∼200 mL of H2O, stirring constantly. Adjust the pH to 7.3 using CsOH solution. Measure the osmolarity of the CsF/CsCl recording solution
using an osmometer. Adjust to ∼300 mOsm by adding H2O. Verify the pH after adjustment of osmolarity, adding CsOH or HCl if necessary. Store the solution at 4°C for up to several
months. (This recording solution is optimized for simple voltage-clamp recordings. Different recording solutions are appropriate
for different experimental goals. QX-314 [2 mm] may be added to block sodium channels and improve clamp.)
|
