Artificial Cerebrospinal Fluid (ACSF) (10×)
| Reagent | Amount to add | Final concentration in 10× ACSF | Final concentration in 1× ACSF |
|---|---|---|---|
| NaCl | 73.05 g | 1.25 m | 125 mm |
| NaHCO3 | 21.843 g | 260 mm | 26 mm |
| NaH2PO3 | 1.725 g | 12.5 mm | 1.25 mm |
| KCl | 1.864 | 25 mm | 2.5 mm |
-
In a 1-L volumetric flask, combine the above reagents. Shake to prevent poorly soluble monobasic crystals from forming. Add MgCl2 from a concentrated stock solution to a final concentration of 10 mm (in 10× ACSF). (For example, add 5 mL of a 2 m MgCl2 stock.) Add H2O to the 1-L line and stir until all reagents are dissolved. Store 10× ACSF in a 1-L flask at 4°C for up to 2 wk. Use this 10× stock solution daily to prepare Artificial Cerebrospinal Fluid (ACSF) (1×). Do not use if crystals form. (10× ACSF can also be prepared without MgCl2 for experiments requiring different calcium concentrations.)
- © 2017 Cold Spring Harbor Laboratory Press
