| F-12 Nutrient Mixture (Ham) with GlutaMAX (Invitrogen 31765-035) |
250 mL |
|
| Iscove’s modified Dulbecco’s medium (IMDM) with GlutaMAX (Invitrogen 31980-030) |
250 mL |
|
| BSAa fraction V (e.g., Equitech-Bio, Inc., BAC62 or Sigma-Aldrich A1470)
|
2.5 g |
5 mg/mL |
| Thioglycerol (11.5 m stock; Sigma-Aldrich M7522)
|
20 µL |
450 µm |
| Insulin (10 mg/mL stock; Roche 11376497001) |
350 µL |
7 µg/mL |
| Transferrin (30 mg/mL stock; Roche 10652202001) |
250 µL |
15 µg/mL |
| Chemically defined lipid concentrate (100× stock; Invitrogen 11905-031) |
5 mL |
1× |
| Store the medium (final 500 mL) at 4°C and use within 1 mo. |
| aBSA may need to be batch-tested.
|
